|
KMID : 0364820110470030194
|
|
Korean Journal of Microbiology
2011 Volume.47 No. 3 p.194 ~ p.199
|
|
|
Characterization of an Alkaline Protease from an Alkalophilic Bacillus pseudofirmus HS-54
|
|
Bang Seong-Ho
Joung In-Sil
|
|
|
Abstract
|
|
|
|
An alkalophilic bacterium producing alkaline protease was isolated from waste water and solar saltern sample and identified as Bacillus pseudofirmus HS-54 based on morphological, biochemical characteristics as well as 16S-rRNA gene sequencing. The HS-54 protease was purified to homogeneity using ammonium sulfate precipitation, DEAE cellulose column chromatography, and sephadex G-100 gel filtration with a 4.0 purification fold. The molecular mass of the purified enzyme was estimated by SDS-PAGE to be 27 kDa. The optimal pH and temperature for the purified protease activity were 10.0 and 50°C, respectively. The purified enzyme was relatively stable at the pH range of 6.0-11.0 and at the temperature below 50°C. This enzyme was activated by Ca2+ and Mg2+ and inhibited by Hg2+, Cu2+, Zn2+, Al3+, Ag2+. And this enzyme was strongly inhibited by PMSF, suggesting that it belongs to the serine protease superfamily.
|
|
|
KEYWORD
|
|
|
Bacillus pseudofirmus HS-54, alkaline protease, purification, serine protease
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|